Fig. 2: SP-1 activates LINC01016 transcription and facilitates the migration and invasion of GC cells. | Cell Death & Disease

Fig. 2: SP-1 activates LINC01016 transcription and facilitates the migration and invasion of GC cells.

From: SP-1-activated LINC01016 overexpression promotes gastric cancer invasion and metastasis through inhibiting EIF4A3-mediated MMP9 mRNA decay

Fig. 2

A Fragments of the LINC01016 promoter are cloned into the pGL3-basic vector upstream of firefly luciferase. (pGL3-1000, pGL3-750, pGL3-500, pGL3-250, pGL3-125) B Luciferase activity assays disclose that the promoter activity is significantly decreased from pGL3-500 to pGL3-250 and pGL3-125 to pGL3-0. C SP-1 and E2F-1 overexpression lead to a significant increase in luciferase activity. D qRT-PCR indicates that SP-1 overexpression promotes the expression of LINC01016 in GC cells. E The JASPAR website analyzes the potential SP-1 binding sites in the LINC01016 promoter (from −434 to −43 bp), divided into four regions. F Four pairs of primers are constructed to cover the SP-1 binding region, all of which could amplify PCR products from the immunoprecipitated DNA fragments of anti-SP-1 antibody, especially primer 4. G LINC01016 binding site mutants exhibite reduced luciferase activity. Data are presented as means ± SEM, from three independent experiments. *P < 0.05, **P < 0.01,***P < 0.001.

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