Fig. 5: Overexpression of ELF3 in PTEN-deficient Human and Murine Lung Epithelium Induces the Expression of Ferroptosis Inhibitor SLC7A11. | Cell Death & Disease

Fig. 5: Overexpression of ELF3 in PTEN-deficient Human and Murine Lung Epithelium Induces the Expression of Ferroptosis Inhibitor SLC7A11.

From: Overexpression of ELF3 in the PTEN-deficient lung epithelium promotes lung cancer development by inhibiting ferroptosis

Fig. 5

A Transcriptomic analysis of lung tissues from WT (n = 3) and Ptend/dELF3OV/+ (n = 3). DEGs represent the differentially expressed genes from Ptend/dELF3OV/+ vs WT. B Conducting pathway enrichment analysis of DEGs from Ptend/dELF3OV/+ vs WT by KEGG. C Venn diagrams indicate the strategy to select DEGs related to tumorigenesis and inhibition of ferroptosis from three groups, Ptend/dELF3OV/+ vs WT, Ptend/dELF3OV/+ vs WT and Ptend/dELF3OV/+ vs WT. D Joint analysis of 4 groups of transcriptomic data of mouse models, showing the expression profiling of all genes in 4 groups (12 samples), Wild-type, ELF3OV/+, Ptend/d and Ptend/dELF3OV/+. E, F Comparative analysis of SLC7A11 expression levels in lung tissues of WT, ELF3OV/+, Ptend/d and Ptend/dELF3OV/+ mice through IHC staining and RT-qPCR. The 2−ΔΔCt method was used to determine the relative expression levels of Slc7a11 mRNA. n = 3 (three independent experiments). Statistical comparisons were performed using the one-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001. G Expression levels of SLC7A11 in NL20 PTEN−/− and PTEN−/−ELF3ov cells were detected by RT-qPCR and WB. The 2−ΔΔCt method was used to determine the relative expression levels of SLC7A11 mRNA. n = 3 (three independent experiments). Statistical comparisons were performed using the Unpaired t-test, *p < 0.05, **p < 0.01, ***p < 0.001. Error bars represent the SEM. H ELF3 motif is detected at the promoter region of SLC7A11. I, J Left: Verification of the binding frequency of ELF3 at the SLC7A11 promoter region through ChIP-qPCR. Right: Detecting the regulatory relationship between ELF3 and SLC7A11 through dual-luciferase reporter gene assay. K The correlation between ELF3 and SLC7A11 expression in lung cancer patients with low PTEN expression (PMID: 29625048). L The correlation between SLC7A11 expression levels and overall survival in lung cancer patients with low PTEN expression and high expression of ELF3 (PMID: 29625048).

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