Fig. 6: Erastin, targeting SLC7A11, significantly induces ferroptosis of lung cancer cells with ELF3 overexpression and PTEN-deficient background. | Cell Death & Disease

Fig. 6: Erastin, targeting SLC7A11, significantly induces ferroptosis of lung cancer cells with ELF3 overexpression and PTEN-deficient background.

From: Overexpression of ELF3 in the PTEN-deficient lung epithelium promotes lung cancer development by inhibiting ferroptosis

Fig. 6

A Cell proliferation capacity of H1650 PTENnull and PTENnullELF3ov cells after erastin treatment measured using cell colony formation assay. n = 3 (three independent experiments). B Quantitative analysis of cell colony formation of H1650 PTENnull and PTENnullELF3ov cells treated with erastin on the first and fifth days using ImageJ. Statistical comparisons were performed using the 2-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001. Error bars represent the SEM. C Cell proliferation capacity of NL20 PTEN−/− and PTEN−/−ELF3ov cells after erastin treatment using cell colony formation assay. n = 3 (three independent experiments). D Quantitative analysis of cell colony formation of NL20 PTEN−/− and PTEN−/−ELF3ov cells treated with erastin on the first and fifth days using ImageJ. Statistical comparisons were performed using the 2-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001. Error bars represent the SEM. MDA (E) and GSH (F) assays of H1650 PTENnull and PTENnullELF3ov cells after five days of erastin treatment. μM/μg protein represents the level of MDA/GSH. n = 3 (three independent experiments). Statistical comparisons were performed using the Unpaired t-test, *p < 0.05, **p < 0.01, ***p < 0.001. Error bars represent the SEM. MDA (G) and GSH (H) assays in NL20 PTEN−/− and PTEN−/−ELF3ov cells after five days of erastin treatment. μM/μg protein represents the level of MDA/GSH. n = 3 (three independent experiments). Statistical comparisons were performed using the Unpaired t-test, *p < 0.05, **p < 0.01, ***p < 0.001. Error bars represent the SEM. I–L The volume of the xenograft tumors was measured and quantified. Each group consists of five nude mice (n = 5). Statistical comparisons were performed using the 1-way & 2-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001. Error bars represent the SEM.

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