Fig. 3: CPNE7 knockdown inhibits the growth and metastasis of CRC cells in vivo.
From: CPNE7 promotes colorectal tumorigenesis by interacting with NONO to initiate ZFP42 transcription
A shControl and shCPNE7 HCT116 cells were injected into BALB/c-nude mice (n = 5 mice per group). Volume changes of xenograft tumors in nude mice are shown on the left, and mice images are shown on the right. B Tumor images are shown on the left, and statistical analysis for tumor weights is shown on the right. C Expression of CPNE7 in xenograft tumors were detected by Western blot. D IHC staining of Ki-67 in xenograft tumors in (B). Scale bar: 100 μm. E Representative images of the visible metastatic nodules in livers. (n = 4 mice per group). F Representative images of liver (E) histology stained with hematoxylin and eosin are shown on the left, and the statistical analysis is shown on the right. For (A– F), data are shown as mean ± SD and two-tailed unpaired Student’s t-test was used. *p < 0.05, **p < 0.01, ***p < 0.001. Data are representative of at least three independent experiments.
