Fig. 4: RAD51 expression is regulated by miR-124 in GBM cells. | Cell Death & Disease

Fig. 4: RAD51 expression is regulated by miR-124 in GBM cells.

From: Overexpression of miR-124 enhances the therapeutic benefit of TMZ treatment in the orthotopic GBM mice model by inhibition of DNA damage repair

Fig. 4

A Venn plot showing the overlap of four predicted miRNA-mRNA binding datasets (miRcode, miRTarBase, miRDB, TarBase). B Identification of potential target genes of miR-124. Only two genes (RAD51, PRDM13) were screened by overlapping the predicted genes (target score >50) in (A) with significant differential expression genes (LogFC >3, P < 0.05) in TCGA. C Correlation analysis between miR-124 and RAD51 or PRMD13 using TCGA database. A significant negative correlation was shown only for RAD51 and miR-124. P values were calculated using Pearson correlation test. D Quantitative real-time chain reaction (qRT-PCR) analysis of miR-124 expression in GBM cells transfected with miR-Ctrl or miR-124 mimics. E, F Western blot and qRT-PCR analysis of RAD51 expression in multiple GBM cell lines transfected with miR-Ctrl or miR-124 mimics. G Western blot analysis of RAD51 expression in GBM cells transfected with anti-miR-Ctrl or anti-miR-124. H Predication of one binding site of miR-124 on RAD51 3′-UTR by TargetScan. I Dual-luciferase reporter assay showing miR-124 directly targets the RAD51 3′-UTR. Firefly luciferase activity of the reporter was normalized to the internal Renilla luciferase activity. All experiments were repeated at least 3 times. All data are presented as means ± SD. P values were calculated using unpaired Student’s t-test.

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