Fig. 3: Early loss of striatal neuronal PKD in R6/1 mice occurs mainly in neurons while PKD expression appears in astrocytes.
From: Down-regulation of neuroprotective protein kinase D in Huntington´s disease
A Representative confocal microscopy images of PKDT (red channel) in neurons (NeuN+, green channel) in striatum of 3.5-month-old WT and R6/1 mice. Nuclei were stained with DAPI. Scale bar: 20 µm. Graph represents the quantification of PKDT intensity staining in striatal neurons of WT compared to R6/1 mice (3 animals per group). PKDT immunohistochemistry in cerebral cortex (B) and globus pallidum (C) sections from 7.5-month-old WT and R6/1 mice. Scale bar: 150 µm. Graphs represent the quantification of chromogen detection and was expressed as DAB-staining reciprocal intensity (Arbitrary Units, A. U.) (B) or quantification of PKDT-positive astrocytes per area (C) (4 animals per group). Data are represented as mean ± SEM. * P < 0.05 or ** P < 0.01 using unpaired Student’s t test.
