Fig. 4: NEDD4L bound to SLC39A14, SLC39A8, and STEAP3.

A Docking model between NEDD4L and SLC39A14 and surface map of their interface residues. Purple, NEDD4L; sky blue, SLC39A14; green dashed line, hydrogen bond; red dashed line, salt bridge. B Docking model between NEDD4L and SLC39A8 and surface map of their interface residues. Purple, NEDD4L; sky blue, SLC39A8; green dashed line, hydrogen bond; red dashed line, salt bridge. C Docking model between NEDD4L and STEAP3 and surface map of their interface residues. Purple, NEDD4L; sky blue, STEAP3; green dashed line, hydrogen bond; red dashed line, salt bridge. D–F NK-92 cell lysates were incubated with IgG control, and anti-His or -Myc tag antibodies (n = 3). 5% of lysate was used as input control. Cells were transfected with His-tagged SLC39A14 (D), SLC39A8 (E), and STEAP3 (F) constructs, respectively. Schematic representation of ___domain deletion mutants of NEDD4L (G), SLC39A14 (H), SLC39A8 (I), and STEAP3 (J). K, L Co-immunoprecipitation identifying the key domains that determine the binding of NEDD4L and SLC39A14 in NK-92 cells (n = 3). Cells were co-transfected with His-tagged SLC39A14 and Myc-tagged NEDD4L WT or deletion mutants (K), or Myc-tagged NEDD4L and His-tagged SLC39A14 WT or deletion mutants (L). M, N Co-immunoprecipitation identifying the key domains that determine the binding of NEDD4L and SLC39A8 in NK-92 cells (n = 3). Cells were co-transfected with His-tagged SLC39A8 and Myc-tagged NEDD4L WT or deletion mutants (M), or Myc-tagged NEDD4L and His-tagged SLC39A8 WT or deletion mutants (N). O, P Co-immunoprecipitation identifying the key domains that determine the binding of NEDD4L and STEAP3 in NK-92 cells (n = 3). Cells were co-transfected with His-tagged STEAP3 and Myc-tagged NEDD4L WT or deletion mutants (O), or Myc-tagged NEDD4L and His-tagged STEAP3 WT or deletion mutants (P). WT wild-type.