Fig. 2: VP1 recruited neutrophils into the lung.
From: Neutrophil extracellular traps induced by VP1 contribute to pulmonary edema during EV71 infection
The single-cell suspensions were obtained from the lungs of mice from each experimental group and stained with anti-CD45-FITC, anti-Ly6G-PE, and anti-F4/80-APC antibodies. Flow cytometric analysis was performed and representative flow cytometry pseudocolor plots gated on neutrophils (CD45+Ly6G+) and macrophages (CD45+F4/80+) were shown. a, b The percentage of neutrophils in the lung was markedly increased in VP1-treated mice. c, d The percentage of macrophages in the lung was slightly increased in VP1-treated mice. Values are expressed as the mean ± SEM. n = 4–6 per group. *p < 0.05, **p < 0.01, ***p < 0.0001
