Fig. 3: VP1 increased neutrophil chemokines in lung tissue at both the RNA and protein levels.
From: Neutrophil extracellular traps induced by VP1 contribute to pulmonary edema during EV71 infection
Real-time PCR assays were performed to detect the mRNA expression levels of the neutrophil chemokines a–c KC, CXCL2, and CXCL5 and the macrophage chemokines e–g MCP-1, CCL3, and CCL4. ELISA assays were performed to detect the protein levels of d KC and h MCP-1 in lung homogenates. Data are presented as the mean ± SEM. n = 3–5 for each group. *p < 0.05, **p < 0.01, ***p < 0.0001
