Fig. 8: The effect of knockdown of c-Myc or KLF4 on the proliferation of the self-renewing macrophages.
From: Establishment of bone marrow-derived M-CSF receptor-dependent self-renewing macrophages
a The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and analyzed for the expression of c-Myc by qRT-PCR. The level of c-Myc is shown, by setting the value of the control (transfection with Cr pool-A siRNA) as 100%. b The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and analyzed for the expression of KLF4 by qRT-PCR. The level of c-Myc is shown, by setting the value of the control (transfection with Cr pool-A siRNA) as 100%. c The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and subjected to the MTT assay. The number of cells is shown, by setting the value of the control (transfection with Cr pool-A siRNA) as 100% (mean ± SD, n = 3), *p < 0.05. d, e The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and subjected to the cell cycle analysis. In e, the percentage of cells in the S phase is summarized (mean ± SD, n = 3), *p < 0.05. f, g The self-renewing macrophages were transfected with siRNAs indicated, cultured for 2 days, and subjected to the apoptotic cell analysis. In g, the percentage of cells positive for both Annexin V and 7-AAD is summarized (mean ± SD, n = 3), *p < 0.05.
