Fig. 4: ABCA8 decreases the chemosensitivity of PC cells to GEM via ERK signaling. | Cell Death Discovery

Fig. 4: ABCA8 decreases the chemosensitivity of PC cells to GEM via ERK signaling.

From: ABCA8-mediated efflux of taurocholic acid contributes to gemcitabine insensitivity in human pancreatic cancer via the S1PR2-ERK pathway

Fig. 4

A The expression levels of phosphorylated ERK (p-ERK) and total ERK (T-ERK) proteins in parental and Gem-R pancreatic cancer cells were analyzed by western blotting (n = 3 independent biological repeats). B Western blot analysis of the effect of ABCA8 knockdown on ERK phosphorylation in Gem-R pancreatic cancer cells (n = 3 independent biological repeats). C Western blot analysis of the effect of ABCA8 overexpression on ERK phosphorylation in PANC-1 and CFPAC-1 cells (n = 3 independent biological repeats). D Evaluation of the effect of the ERK inhibitor SCH772984 (SCH) on GEM sensitivity in ABCA8-overexpressing and control (VC) cells by CCK-8 assays after 72 h of treatment. Data are presented relative to the respective untreated controls for ABCA8-overexpressing and control cells (n = 4 independent biological repeats). E Analysis of the effect of the ERK inhibitor SCH772984 on GEM-induced apoptosis in ABCA8-overexpressing and control cells by flow cytometry (n = 4 independent biological repeats). F Western blot analysis of the effect of the ERK inhibitor SCH772984 on BAX and BCL2 expression in ABCA8-overexpressing and control cells (n = 3 independent biological repeats). ***P < 0.001.

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