Fig. 5: ABCA8 stimulates the efflux of TCA that contributes the GEM resistance in PC cells. | Cell Death Discovery

Fig. 5: ABCA8 stimulates the efflux of TCA that contributes the GEM resistance in PC cells.

From: ABCA8-mediated efflux of taurocholic acid contributes to gemcitabine insensitivity in human pancreatic cancer via the S1PR2-ERK pathway

Fig. 5

A The mRNA expression levels of bile acid biosynthesis enzymes CYP7A1, CYP7B1, and CYP27A1 in pancreatic adenocarcinoma (PAAD) (tumor samples: n = 179; normal samples: n = 171) and liver hepatocellular carcinoma (LIHC) (tumor samples: n = 369; normal samples: n = 160) were analyzed using the online tool from the GEPIA2 website (http://gepia2.cancer-pku.cn) based on TCGA and GTEx database. B Measurement of total BAs in human pancreatic cancer tissues and corresponding adjacent normal tissues (n = 9). C Measurement of the intracellular total BAs of parental and Gem-R PANC-1 and CFPAC-1 cells, and hTERT-HPNE cells (n = 4 independent biological repeats). D Measurement of extracellular TCA in the cell culture medium of ABCA8-overexpressing PANC-1 and CFPAC-1 cells and control cells (VC) by a human TCA ELISA kit (n = 4 independent biological repeats). E Evaluation of the effect of exogenous TCA and/or S1PR2 inhibitor JTE-013 on GEM sensitivity in ABCA8-overexpressing and control (VC) cells by CCK-8 assays after 72 h of treatment (n = 4 independent biological repeats). E Western blot analysis of ERK phosphorylation in PANC-1 and CFPAC-1 cells treated with the indicated concentrations of exogenous TCA (n = 3 independent biological repeats). *P < 0.05, **P < 0.01, and ***P < 0.001.

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