Fig. 6: ABCA8-induced extracellular accumulation of TCA contributes to GEM insensitivity by activating SIRP2/ERK signaling.

A, B Analysis of cell survival by CCK-8 assay (A) or cell apoptosis levels by flow cytometry (B) of ABCA8-overexpressing cells and control cells (VC) in the presence of GEM and/or the S1PR2 inhibitor JTE-013 (n = 4 independent biological repeats). Data are presented relative to the respective untreated controls for ABCA8-overexpressing and control cells (A). C Western blot analysis of the effect of JTE-013 on ERK phosphorylation and the expression of BAX and BCL2 in ABCA8-overexpressing cells and control cells (n = 4 independent biological repeats). *P < 0.05, **P < 0.01, and ***P < 0.001.