Fig. 1: Old Ambra1+/gt mice display reduced RGC survival after ONC.
From: Age related retinal Ganglion cell susceptibility in context of autophagy deficiency
a Immunostaining of retinal ganglion cells in flat mounts at different time points after ONC showing γ-Synuclein (red) and Calbindin (cyan) staining. b % of RGCs throughout a time course up to 10 days after ONC in young Ambra1+/+ (WT) animals. The number of RGCs was calculated by subtracting from the amount of γ-Synuclein positive cells minus the displaced amacrine double stained γ-Synuclein and Calbindin cells. The control eyes are represented as baseline value, n = 4 animals per group. #p < 0.01 in comparison to the corresponding non-injured eye. c % survival of RGCs in 3-months-old (young) Ambra1+/+ and Ambra1+/gt mice 7 days after ONC, n = 4 animals per group. d Graph showing the survival of RGCs in old (13-months-old) Ambra1+/+ and Ambra1+/gt mice 7 days after ONC, n = 8 animals per group. #p < 0.01 e % of RGCs stained with Brn3a in cryosections of old Ambra1+/+ and Ambra1+/gt mice 7 days after ONC, n = 5 animals per group. Data show mean +/− SEM, *p < 0.05.
