Fig. 6: Increased RGC apoptosis and decreased neuronal growth in Ambra1+/gt derived RGCs.
From: Age related retinal Ganglion cell susceptibility in context of autophagy deficiency
a % of apoptotic cells determined by counting condensed nuclei in Ambra1+/+ and Ambra1+/gt retinal cell cultures (n = 4 for WT, n = 5 for HT), #p < 0.01, referring to comparison with WT, Data represent mean +/− SEM. b RGC numbers in culture were determined 1 and 3 DIV using DIC pictures. The graph shows the number of RGCs, n = 15. #p < 0.01, *p < 0.05. Data represent mean +/− SEM. c Representative DIC pictures of the RGCs cultures after 3DIV. The scale bar 25 µm. d % of apoptotic RGCs in culture in Ambra1+/+ and Ambra1+/gt Apoptotic RGCs were counted using DAPI and TUJ1 staining (n = 4 for WT, n = 5 for HT), *p < 0.05, in comparison to the corresponding WT value. e The length of RGC neurites was measured manually in DIC pictures in Ambra1+/+ and Ambra1+/gt. The graph represents the length of neuronal projection that each RGC grows at 1 DIV and 3 DIV, n = 15, *p < 0.05, referring to comparison with corresponding WT value, data represent mean +/− SEM.
