Fig. 5: Desmoplakin negatively regulates the adipogenic differentiation of hAMSCs. | Cell Death Discovery

Fig. 5: Desmoplakin negatively regulates the adipogenic differentiation of hAMSCs.

From: Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway

Fig. 5

a qRT-PCR analysis verified the DSP expression profile at the indicated time points after adipogenic differentiation of hAMSCs. b DSP was silenced in hAMSCs using two independent siRNAs (si-DSP-2 and si-DSP-3). The knockdown efficiency was verified by qRT-PCR (upper) and western blotting (lower) as compared with the negative control (si-NC). c, d Western blot analysis (c) and qRT-PCR analysis (d) of adipogenic markers (PPARγ, AP2, and CEBPα) in DSP knockdown hAMSCs and control hAMSCs on day 3 after adipogenic induction. e, f Oil red O staining of adipose lipids in DSP knockdown hAMSCs and control hAMSCs on day 10 after adipogenic induction (e) and quantification of the oil red O staining (f). GAPDH and ACTB were used as internal controls for western blotting. The quantitative data were normalized to GAPDH, n = 3; data are shown as the mean ± SD; **P < 0.01, ***P < 0.001; scale bars: 200 µm.

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