Fig. 5: CDK12 is involved in the promotion of proliferation and inhibition of apoptosis in trastuzumab-resistant breast cancer. | Cell Death Discovery

Fig. 5: CDK12 is involved in the promotion of proliferation and inhibition of apoptosis in trastuzumab-resistant breast cancer.

From: JWA suppresses proliferation in trastuzumab-resistant breast cancer by downregulating CDK12

Fig. 5

A, B qRT–PCR results showing levels of CDK12 expression in JIMT1 and BT474-Tr cells transfected with CDK12 siRNAs, respectively. Protein levels of PCNA, Cleaved Caspase-3, Bcl-2, BAX in JIMT1 and BT474-Tr cells transfected with CDK12 siRNAs. β-actin served as the internal control. C, D Interference with CDK12 siRNAs in JIMT1 and BT474-Tr cells impacted cell proliferation as determined by colony formation assays. E, F Apoptotic cells increased after knocking down of CDK12 in JIMT1 and BT474-Tr cells. **P < 0.01, ***P < 0.001, ****P < 0.0001.

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