Fig. 5: PIAS1 promotes autophagy of Schwann cells by regulating miR-124-EZH2/STAT3 axis. | Cell Death Discovery

Fig. 5: PIAS1 promotes autophagy of Schwann cells by regulating miR-124-EZH2/STAT3 axis.

From: PIAS1 alleviates diabetic peripheral neuropathy through SUMOlation of PPAR-γ and miR-124-induced downregulation of EZH2/STAT3

Fig. 5

A The mRNA expression of miR-124, PIAS1, EZH2, and STAT3 as determined by RT-qPCR. B The protein expression of PIAS1, EZH2, and STAT3 in Schwann cells treated with oe-PIAS1 alone or in combination with miR-124 mimic, as determined by western blot assay. C The mRNA expression of miR-124, PIAS1, EZH2, and STAT3 in response to PIAS1 overexpression alone or in combination with EZH2/STAT3 upregulation, as determined by RT-qPCR. D The protein expression of PIAS1, EZH2, and STAT3 in response to PIAS1 overexpression alone or in combination with EZH2/STAT3 upregulation, as determined by western blot assay. E The number of LC3-II particles in cells with different treatment as detected by immunofluorescence staining. F The proportion of apoptosis of cells with different treatment as detected by flow cytometry. G The expression of apoptosis and autophagy-related proteins in response to PIAS1 overexpression alone or in combination with EZH2/STAT3 upregulation, as determined by western blot assay. *p < 0.05. Data between two groups were compared by independent sample t test, and those among multiples by one-way ANOVA, followed by Tukey’s post hoc tests. The experiment was conducted in triplicates.

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