Fig. 4: DHA induces ferritinophagy in AML cells. | Cell Death Discovery

Fig. 4: DHA induces ferritinophagy in AML cells.

From: Dihydroartemisinin-induced ferroptosis in acute myeloid leukemia: links to iron metabolism and metallothionein

Fig. 4

a Immunoblotting analysis of LC3, NCOA4 and FTH1 in MOLM-14 cells treated with, FAC 10 µM, CQ 10 µM, DHA 5 µM or combination of FAC + DHA or CQ + DHA for 12 h. b Immunofluorescence analysis of FTH1 or LAMP2 in MOLM-14 treated with DHA 5 µM, FAC 10 µM or both for 16 h. c Viability curves for the indicated cells at 24 h post treatment with DMSO, chloroquine (10 µM) or VPS-34in1 (5 µM) and DHA at increasing doses. Error bars, ± standard deviation (n = 3). d Immunoblotting analysis of CD71, NCOA4, and FTH1 in MOLM-14 and OCI-AML2 cells treated with, FAC 10 µM, VPS34-In1 5 µM, and DHA 5 µM or combination of FAC + DHA or VPS34-In1 + DHA for 24 h. e Viability curves for MOLM-14 or OCI-AML2 cells transduced with scramble or shNCOA4 treated with DHA at increasing doses for 24 h and corresponding IC50%. Error bars, ± standard deviation (n = 3).

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