Fig. 1: Construction of weighted gene co-expression network and clinical and functional analysis of KLF10.

A Heatmap with hierarchical clustering dendrogram of kidney samples in GSE1563. B Negative correlation between Epithelial Proliferation Score (ProScore) and Log₂ (Serum Creatinine). C Analysis of appropriate soft-thresholding powers for WGCNA. D Average network connectivity under weighting coefficients of WGCNA. E Clustering dendrograms of genes in 21 samples, from which 10 coexpression modules were constructed with different colors. F The Heatmap plot depicts the TOM among all genes in the analysis, which shows the interactions among co-expression modules. The stronger intensity of orange indicates greater overlaps. G Interaction network analysis based on WGCNA and network of KLF10 with its closely interacted genes. Each dot is color-coded by its corresponding module. H Histogram of functional enrichment analysis of genes closely interacted with KLF10. I Bubble plot of the relationship between the coexpression module and clinical data. The size shows the corresponding -log₁₀ (P value) and the color represents the correlation coefficient. J Bubble plot of the relationship between the KLF family and clinical data with histogram displaying the number of closely interacted genes with each KLF. The size shows the corresponding -log₁₀ (P value) and the color represents the correlation coefficient. K Negative correlation between KLF10 and ProScore/Log₂ (Serum Creatinine). L Scatter plot of relative expression of KLF10 in well function group versus acute dysfunction group. M ROC curve with AUC value of KLF10. N Scatter plot of tubulointerstitial relative expression of KLF10 in control groups versus renal disease groups. ***p < 0.001 and ****p < 0.0001 vs control group at the same experimental conditions.