Fig. 3: Relationship between BAP1 and CAMK2D expression in MMe tissues.

a Assessment of CAMK2D mRNA expression status in MMe patients with or without BAP1 mutation using a public database. The raw fluorescence intensity representing CAMK2D expression in the specimens from BAP1-intact and BAP1-mutant MMe patients is shown. A public dataset ID (TCGA-MESO.htseq fpkm-uq.tsv) of specimens from BAP1-mutant patients was obtained, formatted, and analyzed. Box and whisker plots of the relative gene expression levels of CAMK2D in BAP1-intact (BAP1-Intact, n = 58; blue) and BAP1-mutated (BAP1-Mut, n = 23; red) MMe patients; the middle line and the error bars indicate the mean and standard deviation, respectively. Asterisks indicate significant differences between BAP1-intact and BAP1-mutated patients (*P < 0.05). b, c Representative results of immunohistochemical (IHC) analysis for BAP1 (left panels) and CAMK2D (right panels) expression in a normal mesothelial tissue (b) and MMe tissues from cases #25 and #29 (c). d Summary of IHC results for BAP1 and CAMK2D expression in MMe tissues. Immunoreactivity was independently evaluated by two investigators. Staining intensity was scored as strong (3+), moderate (2+), weak (1+), or negative (0). The bar graph represents the proportion (%) of BAP1-negative or -positive (strong, moderate, and weak) cases with CAMK2D-positive expression (strong, moderate, and weak) in MMe tissues.