Fig. 3: Effect of Prx II on H2O2 induced ROS and Ca2+ accumulation and mitochondria dependent apoptosis in HaCaT cells. | Cell Death Discovery

Fig. 3: Effect of Prx II on H2O2 induced ROS and Ca2+ accumulation and mitochondria dependent apoptosis in HaCaT cells.

From: Depletion of peroxiredoxin II promotes keratinocyte apoptosis and alleviates psoriatic skin lesions via the PI3K/AKT/GSK3β signaling axis

Fig. 3

A, B Detection and quantitation of intracellular ROS by flow cytometry. C, D Detection and quantitation of intracellular Ca2+ by flow cytometry. E, F Detection and quantification of apoptosis in mock and shPrx II HaCaT cells post H2O2 treatment by flow cytometry. G–I Analysis and quantification of expression levels of Prx II and apoptosis-related proteins including Bax/Bcl-2 and cle-Cas3/Pro-Cas3 by western blotting post 6 h treatment with H2O2 in HaCaT cells. The data are represented by the mean ± SEM of three separate samples. (*p < 0.05, **p < 0.01, ***p < 0.001).

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