Fig. 7: Interruption of the STING pathway alleviated NET-induced TF expression in the CLP mouse model. | Cell Death Discovery

Fig. 7: Interruption of the STING pathway alleviated NET-induced TF expression in the CLP mouse model.

From: Neutrophil extracellular traps contribute to immunothrombosis formation via the STING pathway in sepsis-associated lung injury

Fig. 7

Interruption of the STING pathway using H-151 protects against lung injury and coagulation cascades induced by CLP. A H&E staining, Sirius Red staining, α-SMA and fibrinogen immunohistochemical analysis of the lung tissues. Scale bar: 100 μm. B The lung wet/dry ratio (n = 6). C Lung injury was semi quantified according to H&E staining. The lung injury score was recorded (n = 6). D The positive area rate of the α-SMA and fibrinogen in lung tissues were calculated by Fiji/ImageJ software (n = 6). E The mRNA levels of TNF-α, IL-1β, and IL-6 in murine lung tissues (n = 5). F, G PT and APTT were assayed from murine plasma (n = 3). H Western blot images of STING pathway and TF expression in lung tissues. I Representative images of immunohistochemical staining for STING in lung tissues treated with DNase I. Scale bar: 100 μm. J Western blot images of the STING pathway in lung tissues treated with DNase I. Each bar represents the mean ± SD. Statistical analysis for three or more groups was carried out using 1-way ANOVA (B–G). *p < 0.05, **p < 0.01, ***p < 0.001.

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