Fig. 1: IGF2 expression prevents cytotoxicity effect of α-syn PFF in PD cellular models. | Cell Death Discovery

Fig. 1: IGF2 expression prevents cytotoxicity effect of α-syn PFF in PD cellular models.

From: IGF2 prevents dopaminergic neuronal loss and decreases intracellular alpha-synuclein accumulation in Parkinson’s disease models

Fig. 1

A, B SN4741 cells were treated with human recombinant IGF2 (rIGF2) 5 ng/ml or Leu27 5 ng/ml for 1 h. and exposed to human α-syn monomers (Mono) or α-syn PFF. PBS 1× was used as a control. After 24 h, cell death was measured by Sytogreen (A) and LDH assay (B). FC correspond to fold change. C Primary culture of cortical neurons was treated at 6 DIV with rmIGF2 100 ng/mL 1 h previously to incubation with mouse α-syn monomers and α-syn PFF. PBS 1× was used as a treatment control. After 72 h of incubation, cellular damage was evaluated by LDH released assay. D SHSY5Y cells were treated with human recombinant IGF2 (rIGF2) 5 ng/ml for 1 h and exposed to human α-syn monomers and α-syn PFF. After 24 h, cell death was measured by quantification of PI-positive cells. E, F SHSY5Y cells were transfected with IGF2-containing plasmid to overexpress IGF2 (IGF2 OE) or empty vector (EV) as control. After 24 h cells were incubated with human α-syn monomers or α-syn PFF and IGF2 receptor antibody (IGF2 OE + Ab IGF2R). Followed 24 h, cell viability was measured by cresyl violet staining (E) and by quantification of PI-positive cells (F). Scale bar 100 um. Data are presented as mean and SEM of at least three independent experiments. Statistically significant differences detected by two-tailed unpaired t test (**p < 0.01).

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