Fig. 5: Analysis of iCDM-specific signaling pathway on gene expression in HBV-infected PXB cells. | Cell Death Discovery

Fig. 5: Analysis of iCDM-specific signaling pathway on gene expression in HBV-infected PXB cells.

From: A small molecule iCDM-34 identified by in silico screening suppresses HBV DNA through activation of aryl hydrocarbon receptor

Fig. 5

A Schematic of the experimental design of HBV infection and IFN-α and iCDM-34 treatments prior to microarray analysis. PXB cells were infected with HBV genotype C for 1 day (blue) and cultured for 24 days. The cells were then treated with 1000 IU/mL IFN-α or 0.0003–30 μM iCDM-34 for 7 days (orange). The black arrows show the times at which the medium was changed. After the treatments, pgRNA and OAS1 mRNA expression levels were measured by qPCR. Error bars indicate SD (n = 3). **p < 0.01 (two-tailed t test). B Score plot of principal component analysis on microarray-based gene expression profiling of PXB cells treated with vehicle control DMSO, 0.3 μM and 30 μM iCDM-34, or 1,000 IU/mL IFN-α for 7 days (upper panel in C). Comparison of differentially expressed genes in PXB cells with treatment with 1,000 IU/mL IFN-α or 30 μM iCDM-34 for 7 days with an absolute fold-change > 2 and p < 0.05 relative to the vehicle control DMSO (lower panel in C). D Canonical pathway analysis of differentially expressed genes induced by 1,000 IU/mL IFN-α or 30 μM iCDM-34 treatment using the knowledge-based functional analysis software Ingenuity Pathways Analysis (IPA). E Upstream regulator analysis of AhR using IPA with differentially expressed genes specifically induced by 30 μM iCDM-34 treatment.

Back to article page