Fig. 5: A-485 suppresses MLL-r-BET complex attaching to the H3K27Ac to down-regulate CDK/RB/E2F-pathway. | Cell Death Discovery

Fig. 5: A-485 suppresses MLL-r-BET complex attaching to the H3K27Ac to down-regulate CDK/RB/E2F-pathway.

From: The epigenetic regulators EP300/CREBBP represent promising therapeutic targets in MLL-rearranged acute myeloid leukemia

Fig. 5

A The western blot of H3K27Ac and H3 modification of MLL-r cell lines treated with different dose of A-485. B The western blot of H3K27Ac of MLL-r cell lines after knocking out EP300 and CREBBP. C Gene tracks of H3K27Ac signals in E2F-pathway-related genes regions of THP-1 treated with A-485 and DMSO. D The bar plot of the proliferative cells in MLL-r cell lines after knocking out AF9. E The proliferation of MLL-r cell lines knocking out AF9 treatment with A-485. F Bar plot of proliferative cells of MLL-r cell lines treatment with JQ-1. G Western blot of the E2F-pathway-related proteins in MLL-r cell lines treated with different concentration of JQ-1. H Western blot of E2F-pathway-related proteins in MLL-r cell lines treated with DMSO, A-485, JQ1 and combination of A-485 and JQ-1. I The proliferation of MLL-r cell lines treated with DMSO, A-485, JQ-1 and combined A-485 and JQ-1. J Model of how A-485 to inhibit proliferation of MLL-r cell. MLL-r cell lines including THP-1 and Molm13. NS: No Significance.

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