Fig. 3: Transcriptome analysis of the neutrophils isolated from thyroid cancer and normal tissues.

A Scatterplot of PC1 and PC2 from principal-component analysis (PCA) showing clusters in isolated neutrophils from whole blood of thyroid cancer patients (n = 11) and healthy controls (HC, n = 9). Color and shape of points indicate prognosis and diagnosis, respectively. B Heatmap showing hierarchical clustering and gene annotation of 2000 differentially expressed genes in neutrophils isolated from whole blood of thyroid cancer patients and healthy control volunteers. C Differentially enriched Gene Ontology Biological Process (GOBP) pathway from GSEA between isolated neutrophils from whole blood of DTC (n = 9) and UTC (n = 2). Horizontal axis and color represent normalized enrichment score (NES) and p-value, respectively. D Significantly enriched GOBP pathway between neutrophils from whole blood of Stage I–II (n = 7) and Stage IV (n = 4) patients. The horizontal axis and color represent NES and p-value, respectively. E Significantly enriched T-cell related Gene Ontology Biological Process (GOBP) pathway from GSEA between neutrophils obtained from whole blood of DTC (n = 9) and UTC (n = 2). The horizontal axis and color represent normalized enrichment score (NES) and p-value, respectively. F Significantly enriched T-cell related Gene Ontology Biological Process (GOBP) pathway from neutrophils obtained from whole blood of Stage I–II (n = 7) and Stage IV (n = 4) patients. The horizontal axis and color represent NES and p value, respectively.