Fig. 4: IGF2BP2 inhibited the migration, invasion, and inflammatory reactions of RA-FLSs.
From: IGF2BP2 regulates the inflammation of fibroblast-like synoviocytes via GSTM5 in rheumatoid arthritis
A Western blotting was used to detect IGF2BP2 protein level in RA-FLSs after treatment with LPS. B Western blotting was used to detect IGF2BP2 protein level in RA-FLSs after treatment with IGF2BP2 siRNA. C, D qPCR analysis of the mRNA expression of IL-1β and IL-6 in RA-FLSs after treatment with IGF2BP2 siRNA. E, F Wound healing and transwell invasion assays showed the capacity of migration and invasion of RA-FLSs after treatment with IGF2BP2 siRNA. Scale Bar = 150 µm. G IGF2BP2 protein level in LPS-incubated RA-FLSs after overexpressing IGF2BP2, as detected by western blotting. H, I qPCR analysis of the mRNA expression of IL-1β and IL-6 in LPS-incubated RA-FLSs after treatment with IGF2BP2 overexpression lentivirus. J, K Wound healing and transwell invasion assays showed the capacity of migration and invasion of LPS-incubated RA-FLSs after overexpressing IGF2BP2. Scale Bar = 150 µm. *P < 0.05, **P < 0.01, ***P < 0.001.
