Fig. 3: USP9x controls the stability of PYCR3.
From: Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer

A Number of unique peptides and their coverage obtained in USP9x and PYCR3 from LC-MS/MS analysis on co-immunoprecipitation assay. B Co-immunoprecipitates from (A) resolved on western blot, normal rabbit IgG was used as control. C Ubiquitination assay showing the levels of ubiquitinated PYCR3 in USP9x overexpression vs. control upon treatment with MG132 for 4 h. Bar graph shows quantification of ubiquitinated PYCR3 normalized to total PYCR3 levels and the control. D siRNA-mediated knockdown of USP9x, as well as (E) USP9x inhibition with the specific inhibitor FT709 for 48 h reduced PYCR3 protein levels. F USP9x overexpression elevated PYCR3 protein level. G PYCR3 transcription upon USP9x siRNA-mediated knockdown remained unchanged. All quantifications pooled from 3 independent biological replicates. Error bars, ± SD. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.