Fig. 4: USP9x alters the proline metabolism pathway.
From: Deubiquitinase USP9x regulates the proline biosynthesis pathway in non-small cell lung cancer
A Schematic illustration of PYCR3-mediated proline biosynthesis and proline cycle. B Metabolite levels of arginine, ornithine, and proline upon USP9x knockdown. C Cellular fractionation of NCI-H1975 cells. Tubulin served as a marker for the cytosolic fraction, TOMM40 for the membrane fraction. D Enzymatic activity of G6PDH upon USP9x knockdown (n = 3). E Ratio of metabolite levels of 6-phosphogluconate and glucose-6-phosphate. F NADP + /NADPH ratio (n = 3). G Efficiency of PYCR3 knockdown 48 h post transfection. H The effect of PYCR3 knockdown on mitochondrial oxygen consumption rates (OCR) in NCI-H1975 cells normalized to total protein amount. I Calculations of indicated mitochondrial parameters from (H) (n = 3). J Cell growth measured over 4 days showing the effect of PYCR3 knockdown in NCI-H1975, normalized to siRNA control. K Kaplan–Meier analysis of PYCR3. High expression of PYCR3 is associated with poor overall survival of patients with NSCLC. P-value calculated using Log Rank test. Error bars, ±SD. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.
