Fig. 3: ELFN1-AS1 localizes in the cytoplasm, binds to TAOK1, and does not affect TAOK1 expression. | Cell Death Discovery

Fig. 3: ELFN1-AS1 localizes in the cytoplasm, binds to TAOK1, and does not affect TAOK1 expression.

From: The long noncoding RNA ELFN1-AS1 promotes gastric cancer growth and metastasis by interacting with TAOK1 to inhibit the Hippo signaling pathway

Fig. 3

A lncALTAS predicted ELFN1-AS1 was mainly localized in the cytoplasm. B, C Subcellular localization of ELFN1-AS1 was detected by FISH assay and qRT-PCR in MKN45 and AGS (scale bar: 50 μm). D RNA pulldown assay and silver staining assay were applied to find ELFN1-AS1 binding protein, and the result was validated by western blot. E The top 10 proteins that bind to ELFN1-AS1. F The secondary mass spectrometry of TAOK1 protein. G RIP assay showed that ELFN1-AS1 could be enriched by TAOK1 in cell lysates of MKN45 and AGS. H, I ELFN1-AS1 did not affect TAOK1 expression at either the mRNA or protein levels.

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