Fig. 4: TAOK1 inhibits the proliferation, migration, invasion, and metastasis of GC. | Cell Death Discovery

Fig. 4: TAOK1 inhibits the proliferation, migration, invasion, and metastasis of GC.

From: The long noncoding RNA ELFN1-AS1 promotes gastric cancer growth and metastasis by interacting with TAOK1 to inhibit the Hippo signaling pathway

Fig. 4

A, B Detection of knockdown and overexpression efficiency in MKN45 and AGS with qRT-PCR and western blot. C, D The growth curves detected by CCK-8 assay after knockdown or overexpression of TAOK1 in MKN45. E, F Cloning formation assay was applied to evaluate the effect of knockdown of TAOK1 on the proliferation of MKN45. G, H The proliferative capacity of MKN45 was evaluated by EdU assay (scale bar: 100 μm). I, J Wound healing assay in MKN45 with knockdown or overexpression of TAOK1 to evaluate migration ability (scale bar: 100 μm). K, L Transwell assays were used to detect migration and invasion ability in MKN45 with knockdown or overexpression of TAOK1(scale bar: 200 μm). M Xenograft tumors of TAOK1 knockdown or control group. N, O Tumor size and weight of different groups. P Ki-67 expression was detected by immunohistochemical staining (scale bar: 50 μm). Q, R Representative images of lung metastasis and HE staining of the specimen (scale bar: 100 μm). **p < 0.01, ***p < 0.001.

Back to article page