Fig. 6: ELFN1-AS1 inhibits the phosphorylation of STK3 by TAOK1, thereby inhibiting Hippo pathway activation. | Cell Death Discovery

Fig. 6: ELFN1-AS1 inhibits the phosphorylation of STK3 by TAOK1, thereby inhibiting Hippo pathway activation.

From: The long noncoding RNA ELFN1-AS1 promotes gastric cancer growth and metastasis by interacting with TAOK1 to inhibit the Hippo signaling pathway

Fig. 6

A CatRAPID predicted possible fragments of ELFN1-AS1 binding to TAOK1. B RNA pulldown assay was used to detect the binding ability of ELFN1-AS1 after deletion of the corresponding fragment compared to full-length ELFN1-AS1 to TAOK1. C, D The full-length or truncated forms of flag-labeled recombinant TAOK1 protein were incubated with HEK293T cell lysates, and then the enrichment of ELFN1-AS1 was detected by qRT-PCR. E RNA pulldown assays were conducted using ELFN1-AS1-specific probes against full-length or truncated Flag-tagged recombinant TAOK1 protein forms. F Bioinformatics predicted the protein network interacting with TAOK1. G COIP assay was used to verify the interaction between TAOK1 and STK3 and the effect of overexpression of ELFN1-AS1 on the interaction between them. H Effect of altered ELFN1-AS1 expression on the expression levels of various effector molecules in the Hippo signaling pathway. I, J Effect of altered ELFN1-AS1 expression on the level of YAP1 distribution in the nucleus and cytoplasm of cells. ****p < 0.0001.

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