Fig. 2: SPOCD1 could regulate the ferroptosis of KFs.
From: IFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A
A the cck-8 assay of KFs after treated with si-SPOCD1 and erastin(5 μM) for 72 h. B–D the gene expression of TGF-β1, col1 and col3 of KFs after treated with si-SPOCD1 and erastin (5 μM) for 24 h. E Fluorescence intensity detection of ROS of KFs after treated with si-SPOCD1 and erastin(5 μM) for 24 h. F Flow analysis of ROS positive cells of KFs after treated with si-SPOCD1 and erastin (5 μM) for 24 h. G Quantitative statistics of ROS positive cells. H Quantitative statistics of MDA. Bar = 100 μm.*p < 0.05, **p < 0.01, ***p < 0.001.
