Fig. 3: Keloid are impacted by DNA methylation and so as the SPOCD1.
From: IFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A
A Heatmap of hierarchical clustering analysis for samples according to DMPs. B The distribution of keloid’s DNA methylation sites. C The GO function enrichment analysis of these differentially expressed genes regulated by DNA methylation, showing the top 16 enrichment results. D KEGG enrichment results for significantly expressed genes with altered DNA methylation in keloid, showing the top 15 enrichment results. E The CCK-8 assay of KFs after treated with SGI-1027(5 μM) for 5 h. F The expression of protein SPOCD1 and DNMT3A after treated with si-DNMT3A or oe-DNMT3A for 72 h. G Western blotting results of DNMT3A in KFs. H Ion strength of protein mass spectrometry analysis. I The ion strength of SPOCD1 as determined by mass spectrometry analysis. *p < 0.05, **p < 0.01, ***p < 0.001.
