Fig. 6: IFNγ could regulate the ferroptosis of KFs through SPOCD1.
From: IFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A
A Fluorescence intensity detection of ROS of KFs after treated with si-IFNγ, si-SPOCD1 or si-IFNγ + SPOCD1 for 48 h. B Flow analysis of ROS of KFs after treated with si-IFNγ, si-SPOCD1 or si-IFNγ + SPOCD1 for 48 h. C Quantitative statistics of ROS positive rate. D) Electron microscopy detection results of KFs treated with si-IFNγ, si-SPOCD1 or si-IFNγ + SPOCD1 for 72 h. E The western blotting results of KFs after treated with si-IFNγ, si-SPOCD1 or si-IFNγ + SPOCD1 for 72 h. Blue bar=2 μm, Green bar=500 nm, Orange bar=50 μm.*p < 0.05, **p < 0.01, ***p < 0.001.
