Fig. 5: Rescue of oocyte re-awakening failure in Exoc1-G-cKO mice using a PTEN inhibitor.

A Macroscopic Haematoxylin and Eosin-stained images of PTEN inhibitor bpV-treated Exoc1-G-cKO (bpV-Exoc1-G-cKO) mice ovaries. Scale bar = 500 μm. B Representative primary follicles of each mouse genotype (10 weeks old). The control mice were Exoc1+/flox::Gdf9+/Cre, referred to as Exoc1-G-ctrl. Scale bar = 50 μm. C Plots of the oocyte sizes in primary follicles of each mouse genotype at 10 weeks of age. n = 3, one-way analysis of variance. Oocyte diameters in primary follicles in bpV Exoc1-G-cKO mice were significantly longer than those in Exoc1-G-cKO mice. In the bpV Exoc1-G-cKO group, enlarged oocytes appeared in primary follicles (plots circled by dashed line), which were absent in the control group.