Fig. 7: Downstream pathways of c-KIT and growth differentiation factor-9 (GDF9). | Cell Death Discovery

Fig. 7: Downstream pathways of c-KIT and growth differentiation factor-9 (GDF9).

From: Exocyst complex component 1 (Exoc1) loss in dormant oocyte disrupts c-KIT and growth differentiation factor (GDF9) subcellular localization and causes female infertility in mice

Fig. 7

A Representative immunofluorescence images of 16-week-old Exoc1-D-cKO (Exoc1flox/flox::Ddx4+/CreERT2) and Exoc1-D-ctrl (Exoc1flox/+::Ddx4+/CreERT2) primary follicles. FOXO3a signals concentrated in the nucleus of Exoc1-D-cKO oocytes in primary follicles. Scale bar = 10 µm. B The signal intensity of FOXO3a in the nucleus of oocytes in primary follicles. n = 3, Student’s t test. C Representative PCNA and FOXL2, a marker of granulosa cells, co-immunofluorescence images of 16-week-old Exoc1-D-cKO and control primary follicles. Scale bar = 10 µm. D The percentage of PCNA-positive granulosa cells in the primary follicles of Exoc1-D-cKO mice was significantly lower than that in the control. n = 3, Student’s t test.

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