Fig. 2: LMNB2 can promote tumor growth by inducing T-cell apoptosis.
From: LMNB2-mediated high PD-L1 transcription triggers the immune escape of hepatocellular carcinoma
A WB verified the overexpression and knockdown efficiency of LMNB2 in Hepa1-6 cells. B Schematic representation of xenograft tumors with sh-NC + EV, sh-NC + LMNB2, and sh-LMNB2 + EV. C Weight of xenograft tumors of sh-NC + EV, sh-NC + LMNB2, and sh-LMNB2 + EV. D Volume of xenograft tumors of sh-NC + EV, sh-NC + LMNB2, and sh-LMNB2 + EV. E IHC staining of LMNB2, CD3, and CD8 in sh-NC + EV, sh-NC + LMNB2, and sh-LMNB2 + EV in xenograft tumors. Scale bar, 200 μm. F IHC staining scores for LMNB2, CD3, and CD8 in sh-NC + EV, sh-NC + LMNB2, and sh-LMNB2 + EV in xenograft tumors. G WB verified the overexpression and knockdown efficiency of LMNB2 in Huh7 and HepG2 cell lines. H Apoptosis of Jurkat cells co-cultured with Huh7/HepG2 cells treated with sh-NC + EV, sh-NC + LMNB2, or sh-LMNB2 + EV was detected by flow cytometry. I Statistical analysis of apoptotic levels in Jurkat cells (H). J The cell cycle of Jurkat cells co-cultured with Huh7/HepG2 cells treated with sh-NC + EV, sh-NC + LMNB2, and sh-LMNB2 + EV was analyzed by flow cytometry. K Cell cycle statistics of Jurkat cells (J). L–O The levels of IL-2, IL-4, IL-10, and IFN-γ produced by Jurkat cells were detected using ELISA. Data are shown as mean ± SD (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
