Fig. 4: SPOP promotes LMNB2 lysosome-mediated degradation through K29-linkage ubiquitination.
From: LMNB2-mediated high PD-L1 transcription triggers the immune escape of hepatocellular carcinoma
A Coomassie blue staining for the enrichment of LMNB2. B Mass spectrometry of related proteins in LMNB2 enrichment. C and D Interaction between LMNB2 and SPOP. E Interaction between LMNB2 and SPOP in vitro. F Interaction between LMNB2 and SPOP-WT, SPOP-ΔMATH, SPOP-ΔBTB, and SPOP-ΔNLS. G Mode diagram of binding ability between LMNB2 and SPOP-WT, SPOP-ΔMATH, SPOP-ΔBTB, or SPOP-ΔNLS. H Co-localization of LMNB2, SPOP-WT, SPOP-ΔMATH, SPOP-ΔBTB, and SPOP-ΔNLS. Scale bar, 50 μm. I–K SPOP promotes the protein expression of LMNB2. L and M Effect of SPOP on the half-life of LMNB2. N Identifying degradation pathway of LMNB2 induced by SPOP. O Effects of SPOP-WT, SPOP-ΔMATH, SPOP-ΔBTB, and SPOP-ΔNLS on LMNB2 degradation. P Effects of SPOP-WT, SPOP-ΔMATH, SPOP-ΔBTB, and SPOP-ΔNLS on LMNB2 ubiquitination. Q and R Identification of ubiquitination linkage of LMNB2 induced by SPOP. Data are shown as mean ± SD (n ≥ 3). ***p < 0.001.
