Fig. 5: SPOP-induced immune surveillance depends on LMNB2‑PD‑L1 axis in Huh7 cells.

A Huh7 cells achieving sh-NC + EV, sh-SPOP + EV, sh-SPOP+sh-LMNB2 + EV, SPOP + LMNB2 + sh-NC, SPOP + LMNB2-△SBC+sh-NC and SPOP-M35L + LMNB2 + sh-NC were co-cultured with Jurkat cells for 24 h after treatment with DMSO or Atezolizumab (10 ng/mL). WB of Huh7 cells in the HCC cell-Jurkat cell co-culture system for the detection of PD-L1 expression levels. All quantitation was normalized to the protein level of GAPDH in the sh-NC + EV group. B Huh7 cells achieving the above treatment were co-cultured with Jurkat cells for 24 h. Flow cytometry analysis of PD-1 binding on Huh7 cell surface. C Statistics of mean fluorescence intensity (MFI) for PD-1 in (B). D Huh7 cells achieving the above treatment were co-cultured with Jurkat cells for 24 h after treatment with DMSO or Atezolizumab (10 ng/ml). Apoptosis levels in Jurkat cells were detected by flow cytometry. E Statistical analysis of apoptotic levels in Jurkat cells (D). F Huh7 cells achieving the above treatment were co-cultured with Jurkat cells for 24 h after treatment with DMSO or Atezolizumab (10 ng/ml). The cell cycles of Jurkat cells were detected by flow cytometry. G Cell cycle statistics of Jurkat cells (F). H–K The levels of IL-2, IL-4, IL-10, and IFN-γ produced by Jurkat cells were detected using ELISA. Data are shown as mean ± SD (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.