Fig. 6: Functional validation of SINE B2 in mouse oocyte meiosis.

a Schematic illustration. Control and mutant fully grown GV oocytes were injected with a pool of four gapmers targeting the consensus sequence of all B2 SINE elements and cultured in M16 supplemented with Milrinone to prevent GVBD. Oocytes were collected at 24-h postinjection for single cell Q-PCR to measure knockdown efficiency. In different experiments, after 24-h culture in M16 supplemented with Milrinone, injected oocytes were released from Milrinone for 16-h to access GVBD efficiency by DAPI staining. b A box plot shows transcript level of B2 SINE in control and mutant oocytes injected with a pooled gapmers targeting B2 SINE sequence. Each black dot represents an individual oocyte. p-value is calculated by two-tailed Student’s t-test. c Representative confocal microscopy of mutant GV oocytes injected with B2 SINE gapmers with or without GVBD. Chromosome in magenta. Scale bar, 20 µm. d A box plot shows the percentage of control and mutant oocytes undergoing GVBD after injected with a pooled gapmers targeting B2 SINE sequence. Each black dot represents an individual experiment replicate. p-value was calculated by two-tailed Student’s t-test. e Schematic illustration. Control and mutant oocytes were injected with B2 SINE RNAs. Oocytes were culture in M16 medium supplemented with Milrinone for 48-h and released from Milrinone for 16-h to access GVBD efficiency by DAPI staining. f Representative confocal microscopy of wild-type GV oocytes injected with B2 SINE RNAs with or without GVBD. Chromosome in magenta. Scale bar, 20 µm. g A box plot shows the percentage of wild-type GV oocytes undergoing GVBD after injected with either H2O or B2 SINE RNAs