Fig. 6: Single-cell RNA-seq identify the mechanism of action.
From: Maintenance of human haematopoietic stem and progenitor cells in vitro using a chemical cocktail
a T-Distributed Stochastic Neighbor Embedding (t-SNE) analysis identified 11, 3, 4, 8 and 8 subpopulations in 5 samples: uncultured human CD34-positive cells (J1), control cells (J2), cells with the treatment of CFO (J3), HGF (J4) and CFO + HGF (J5). b Dot plot visualization of each subpopulation in the above 5 samples. The size of the dot encodes the percentage of cells within a subpopulation, while the color encodes the average expression level of ‘expressing’ cells. c Expression of selected genes across every subpopulation in the above 5 samples. 100 cells from each subpopulation were randomly sampled three times for gene expression analysis. C, CHIR-99021; F, Forskolin; O, OAC1; HGF, stem cell factor (SCF) and thrombopoietin (TPO)
