Fig. 3: USP7 knockout results in increased DNA methylation in HCT116 cells and mouse embryos. | Cell Discovery

Fig. 3: USP7 knockout results in increased DNA methylation in HCT116 cells and mouse embryos.

From: USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment

Fig. 3

a WB analysis showing the levels of key proteins in DNA methylation and p53 in control and USP7−/− HCT116 cells. b Analysis of DNMT1 and UHRF1 protein stability in control and USP7−/− HCT116 cells. The cells were treated with cycloheximide (100 μg/mL) in culture medium for various times as indicated. The WB data were quantified by ImageJ and shown as relative levels to control (0 h) in the lower panel. c The levels of genomic DNA methylation (mC) in control and USP7−/− HCT116 cells determined by HPLC. ***P ≤ 0.001. d The levels of DNA methylation for each indicated CpG site in LINE1 in control and USP7−/− HCT116 cells determined by bisulfite next-generation sequencing. e Bisulfite sequencing resulted comparing DNA methylation of LINE1 and IAP repetitive elements in control and Usp7−/− mouse embryos.

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