Fig. 5: LPS fails to induce the aggregation of wild-type and mutant caspase-11 CARDs.
From: Crystal structure of caspase-11 CARD provides insights into caspase-11 activation
a, b Multi-angle light scattering (MALS) profile. The black line indicates the experimentally calculated molecular weight. c Purified caspase-11 WT-CARDaa11–101 protein with a TEV recognition sequence between MBP fusion protein and CARD. Protein (1 mg/mL) was incubated with 5 μg LPS at 4 °C for 4 h. d Purified WT-CARDaa11–101 and VLLV-CARDaa11–101 with MBP tag (1 mg each) incubated with 5 μg LPS at 4 °C for 4 h. e, f Purified WT-CARDaa1–101 and VLLV-CARDaa1–101 with MBP tag (1 mg each) incubated with or without 5 μg LPS at 4 °C for 4 h.
