Fig. 6: Definitive human hematopoiesis in early embryonic development.

a t-SNE projection of endothelial and hematopoietic cells, resulting from sub-dividing the cells in Supplementary Fig. S1a as indicated in Supplementary Fig. S1c, assigned based on samples. Each dot represents one cell and colors represent cell samples. The legend shows the number of cells in each sample. b Expressions of known blood and endothelial marker genes at single-cell resolution. Color displays expression level (TPM, Log-scaled). c t-SNE projection of erythroid, definitive HSPC and primitive HPC cluster cells, resulting from sub-dividing the cells in Fig. 6a as indicated in the left panel, according to blood and endothelial marker gene expression as indicated in Fig. 6b. d Expression profiles of selected feature genes in erythroid, definitive HSPC and primitive HPC cluster cells. Each dot represents one cell and color displays expression level (TPM, Log-scaled). e Top Gene Ontology (GO) terms enriched in definitive HSPC and primitive HPC clusters. Colors represent cell clusters. f Violin plots show expression levels (TPM, Log-scaled) of selected myeloid/erythroid, lymphoid and hematopoietic stem/progenitor cell marker genes in definitive HSPC and primitive HPC clusters.