Fig. 8: Natural allele ipa1-2D enhances seedling chilling tolerance.
From: Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice
a Plant morphologies of 2-week-old NILIPA1 and NILipa1-2D seedlings before treatment, after 4 °C treatment for 5 days and subsequent recovery for 6 or 8 days. b Survival rates of NILIPA1 and NILipa1-2D after 4 °C treatment as shown in a. Values are means ± SD (n = 3 biological replicates), and the asterisks indicate significant differences compared with the NILIPA1 (**P < 0.01, Student’s t-test). c Proposed model of OsSAPK6-IPA1-OsCBFs signaling cascade under chilling stress in rice. Under chilling stress, OsSAPK6 phosphorylates IPA1 on Ser201 and Ser213, leading to an accumulation of IPA1 proteins, and IPA1 in turn up-regulates OsCBFs expression by binding to GTAC motifs on the OsCBFs promoter, which then activates the downstream COR genes such as OsCNGC9 to confer chilling tolerance in rice.
