Fig. 6: PHF14 promotes TGF-β-induced systemic metastases of LAD cells in vivo.
From: PHF14 enhances DNA methylation of SMAD7 gene to promote TGF-β-driven lung adenocarcinoma metastasis
a, b TGF-β1-overexpressing A549 and PC9 cells with PHF14 knocked out or corresponding vector-control cells labeled with reporting luciferase were injected via cardiac ventricle into nude mice (n = 5 per group). Representative bioluminescent images of systemic metastasis are shown (a), and quantitation of bioluminescent intensities were analyzed by ROI tools (b). c Metastatic bone lesions in mice intracardially injected with indicated cells were confirmed by micro-CT imaging and H&E staining. d Kaplan-Meier analysis (Log-rank test) of metastasis-free and overall survival of nude mice intracardially injected with indicated TGF-β1-overexpressing A549 and PC9 cells. e, f TGF-β1-overexpressing Calu3 and HCC827 cells labeled with luciferase reporter following indicated treatments were injected via cardiac ventricle into nude mice (n = 5 per group). Representative bioluminescent images of systemic metastasis are shown (e), and quantitation of bioluminescent intensities were analyzed by ROI tools (f). g Metastatic bone lesions in mice intracardially injected with the indicated cells were confirmed by micro-CT imaging and H&E staining. h Kaplan-Meier analysis (Log-rank test) of metastasis-free and overall survival of nude mice intracardially injected with indicated TGF-β1-overexpressing Calu3 and HCC827 cells. TGF-β1, PHF14, SMAD7 or shRNA of DNMT3B were ectopically stably expressed in indicated cell lines by using lentivirus. Scale bar: 100 μm. Error bars represent means ± SD derived from independent experiments. Two-way ANOVA multiple comparison analysis was used for statistical analysis. **P < 0.01; *P < 0.05.
