Fig. 3: Direct contact transmission of pCoV-GD01 in hamsters.

a Each 8- to 10-week-old male hamster that received i.n. inoculation of pCoV-GD01 was placed in an isolated cage, and then the other naïve male animal was cocaged at 24 hpi. b Changes in body weight of hamsters (n = 4). Data are shown as means ± SD. c Throat swabs were collected from the index (n = 4) animals and subjected to viral gRNA analysis. Data are shown as means ± SD. L.O.D, the limit of detection. d, e Viral gRNA and sgRNA in the lungs and tracheas of the index animals were determined by qRT‒PCR at 96 hpi. Data are shown as means ± SD. f IF staining analysis for index hamster lung paraffin sections for pCoV-GD01 (red) at 96 hpi. The white frame was magnified on the right. g Throat swabs were collected from the contact (n = 4) animals and subjected to viral gRNA analysis. Data are shown as means ± SD. h, i Viral gRNA and sgRNA loads in the lungs and tracheas of the contact animals were determined by qRT‒PCR at 96 hpc. Data are shown as means ± SD. j IF staining analysis of paraffin sections of contact hamster tracheas for pCoV-GD01 (red) at 96 hpc. The white frame is magnified below.