Fig. 2: Inhibition of HDAC but not SIRT family deacetylases markedly elevates histone succinylation in various cells. | Cell Discovery

Fig. 2: Inhibition of HDAC but not SIRT family deacetylases markedly elevates histone succinylation in various cells.

From: HDAC1/2/3 are major histone desuccinylases critical for promoter desuccinylation

Fig. 2

a, b WB analysis of histone succinylation by pan-Ksu and site-specific histone Ksu antibodies. HeLa/HCT116/MCF7 cells were treated with different doses of HDAC inhibitor TSA for 12 h (a) or SIRT inhibitor NAM for 24 h (b). c, d WB analysis of succinylated proteins in nuclear, cytosolic, mitochondrial, and histone fractions derived from TSA-treated HeLa cells (c) or SIRT5 KO HeLa cells (d). TSA treatment: 1 μM TSA for 12 h. β-actin, H3, and VDAC as markers for cytosolic, nuclear and mitochondrial fractions, respectively.

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